Role of membrane sulfhydryl groups in rousing of renin secretion
Hypervenom Phantom II FG nike Mercurial Superfly CR7 FG communication: Chun Sik recreation area MD PhD, Split of Physiology, University of Massachusetts school of medicine, 55 Lake path North, Worcester, Ma 01655, U. s,
Picked up 14 May 1990; Revised 19 don’t forget national 1990; Accepted 20 december 1990.
Top of pageAbstractRole of membrane sulfhydryl groups in excitement of renin secretion by sulfhydryl reagents. The present study was designed to address the reactivity and accessibility of the particular class of sulfhydryl groups mixed up in regulatory process of renin secretion. Both mercurial (Like Pchloromercuriphenyl sulfonate[PCMPS]) And therefore nonmercurial sulfhydryl reagents(As an example, 6,6dithiodinicotinic p[DTDN]), Which very slowly pierce the cell membrane of intact cells, Instigated renin secretion. The membrane permeant sulfhydryl reagent Nethylmaleimide had no effect on renin secretion but Nike Mercurial Superfly CR7 FG its membrane impermeant method, Stilbene maleimide, Strongly generated secretion. At the same Hypervenom Phantom II FG time, Disulfide scaling down agents such as dithiothreitol(DTT) Had no effect on renin secretion at low levels, But strongly inhibited it at high levels. Several reagents which are acknowledged to primarily deplete cellular reduced glutathione were without effect on renin secretion. The arousal of renin secretion by PCMPS was rapid in onset, And averted and reversed by DTT and Lcysteine. Likewise, The maximal stimulatory effect of PCMPS has not been addititive to that by diuretics with sulfhydryl reactivity(As an example, Ethacrynic p and mersalyl). The stimulatory effect of PCMPS was not suffering from diuretics which lack sulfhydryl reactivity(Particularly, Bumetanide to furosemide). These results claim that sulfhydryl reagents of both with and without diuretic activity stimulate renin secretion by reacting with specific class of sulfhydryl groups which are readily accessible from the extracellular compartment. Further, These results provide further support the chance that a sulfhydryldisulfide interchange in the membrane may play a regulatory role in the renin secretory process.
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