asics outlet Glucocorticoids modulate renal glucocorticoid receptors and Na

Glucocorticoids modulate renal glucocorticoid receptors and Na

Asics outlet asics store online k ATPase activity

Demetrius Ellis1, Tran Dang Sothi1 and Ellis D Avner1

1Nephrology Laboratory, Children’s Hospital of Pittsburgh, Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA

Correspondence: Demetrius Ellis MD, Director of Nephrology, Children’s Hospital of Pittsburgh, One Children’s Place, 3705 Fifth Avenue at DeSoto Street, Pittsburgh, Pennsylvania 152133417, USA.

Received 16 October 1986; Revised 12 March 1987.

Top of pageAbstractK ATPase activity. Primary cultures of mouse renal tubular epithelial cells were used to study the effect of hydrocortisone on the regulation of glucocorticoid receptors (GR) and on sodium adenosine triphosphatase (NaK ATPase) activity. A GR assay was developed and performed directly on cell monolayers maintained in serum medium to which hydrocortisone at 5 nM, 50 nM, and 5 104 M was added. Compared with control cells grown in medium without hydrocortisone, GR levels per cell decreased by 50% after 48 hours of growth in medium containing 5 nM hydrocortisone. After 96 hours of cell treatment with supraphysiologic hydrocortisone concentrations (50 nM or 5 104 M), GR levels decreased to 28% of control values. In all hydrocortisone treatment groups there was an inverse relation between GR concentrations and NaK ATPase activity. Binding of cell GR by the addition of the antiglucocorticoid RU 38486 in hydrocortisone medium eliminated the glucocorticoid stimulation of NaK ATPase activity. These results demonstrate a time and dose effect of glucocorticoids on GR binding activity and a direct relation between this receptor interaction and NaK ATPase activity in intact renal tubular epithelial cells.

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